Updated November 25, 2009
The most successful programs in men's Division I college basketball history -- ranked by winning percentage, with a minimum of 25 seasons in competition at the Division I level -- through the 2007-08 season:Best Winning Percentage in Division I
| Rank | Team | First Season | Wins | Losses | Ties | Percentage |
| 1 | Kentucky | 1903 | 1988 | 635 | 1 | 0.758 |
| 2 | North Carolina | 1911 | 1984 | 703 | 0 | 0.738 |
| 3 | Kansas | 1899 | 1970 | 793 | 0 | 0.713 |
| 4 | UNLV | 1959 | 1058 | 429 | 0 | 0.711 |
| 5 | Duke | 1906 | 1877 | 817 | 0 | 0.697 |
| 5 | UCLA | 1920 | 1672 | 726 | 0 | 0.697 |
| 7 | Syracuse | 1901 | 1753 | 806 | 0 | 0.685 |
| 8 | Western Kentucky | 1915 | 1602 | 780 | 0 | 0.673 |
| 9 | St. John's | 1908 | 1686 | 868 | 0 | 0.66 |
| 10 | Utah | 1909 | 1637 | 858 | 0 | 0.656 |
| 10 | Louisville | 1912 | 1587 | 831 | 0 | 0.656 |
| 12 | Illinois | 1906 | 1609 | 853 | 0 | 0.654 |
| 13 | Arizona | 1905 | 1568 | 858 | 1 | 0.646 |
| 14 | Notre Dame | 1898 | 1651 | 908 | 1 | 0.645 |
| 15 | Arkansas | 1924 | 1487 | 822 | 0 | 0.644 |
| 15 | Indiana | 1901 | 1641 | 909 | 0 | 0.644 |
| 17 | Missouri State | 1909 | 1498 | 833 | 0 | 0.643 |
| 18 | Connecticut | 1901 | 1499 | 839 | 0 | 0.643 |
| 18 | Temple | 1895 | 1711 | 960 | 0 | 0.641 |
| 20 | Villanova | 1921 | 1505 | 850 | 0 | 0.639 |